From Ground: Start by watering the fivespot plant to dampen the soil which makes removal easier. Next, use a spade or shovel to dig a wide enough trench around the plant, keeping the plant's root ball intact. Once that is done, carefully work the spade under the root ball to lift the plant from its original position.
From Pot: Water the potted fivespot thoroughly before beginning. Turn the pot sideways, while gently tapping its edge on a hard surface. This should loosen the plant and its soil from the pot, allowing you to carefully slide it out while preserving the root ball.
From Seedling Tray: When the fivespot is ready to transplant from a seedling tray, ensure the soil is moist. Push up from the bottom of each cell or gently tease it out from the top without damaging the root system.