From Ground: First, ensure the toothpick cactus plant is hydrated by watering it a day before the planned transplant. This will minimize damage to the roots and keep the plant robust. Then, dig a trench around the toothpick cactus using a trowel, ensuring the plant's root ball stays intact. Carefully work the trowel under the root ball to lift the plant from its original location.
From Pot: Water the toothpick cactus before starting the process, this makes removal easier. Hold the pot on its side and gently tap it to loosen the soil and plant. It should slide out with all the soil attached to its roots.
From Seedling Tray: Water the toothpick cactus seedlings and then carefully use a small fork or your fingers to lift them out, ensuring you're lifting by the leaves and not the stem, as it is fragile.