From Ground: First, water the soil around rough avens to dampen it. Use a garden fork or shovel to gently dig around the plant, starting about 6 inches away from the stem to avoid damaging the roots. Carefully lift the plant, ensuring the root ball remains intact.
From Pot: Water the soil in the pot to make it easier to remove rough avens. Gently squeeze the sides of the pot to loosen the soil. Hold the base of the plant and invert the pot, tapping the bottom to release the root ball. Be careful not to damage the roots during this process.
From Seedling Tray: Water the seedlings before transplanting. Use a small trowel or your fingers to gently lift each seedling from the tray, being careful to keep as much soil around the roots as possible.